Recombinant DNA Lab

Recombinant DNA Analysis

The process of producing recombinant DNA starts with cutting the Plasmid. After cutting it, we taped it into a ring using any order, creating our ring of DNA. Next, we cut the cell DNA out, and taped each strip together. We then used our restriction enzymes to find parts in our rings that matched. When we were done testing the 8 enzymes, whichever one was used in the DNA rings were to get cut. The insulin gene and the plasmid were then “spliced” together. We then tested to see if the plasmid was taken into the host bacteria cells. An example would be ampicillin, as it is an antibiotic that is usually able to kills bacterial cells.

1. Tetracycline, kanamycin, and ampicillin. I would use these three because these usually kill the host bacteria.
2. The restriction enzyme cuts open the plasmid at one site. This is because the DNA will be inserted there. We used Eco R1 and Hin dIII because they were on both of the human genes twice.
3. The DNA won’t be able to be inserted into the plasmid.
4. This process is important in everyday life because this prevents us from contracting illnesses and diseases that would harm us.
5. This process could be used to increase the amount of eggs a chicken produces. This way, we can breed less chickens, kill less chickens, and still get the same amount of eggs. Either that, or we could keep less chickens penned up and only have a few produce a large amount of eggs.