Friday, January 15, 2016

Recombinant DNA Lab

Recombinant DNA Analysis
The process of producing recombinant DNA starts with cutting the Plasmid. After cutting it, we taped it into a ring using any order, creating our ring of DNA. Next, we cut the cell DNA out, and taped each strip together. We then used our restriction enzymes to find parts in our rings that matched. When we were done testing the 8 enzymes, whichever one was used in the DNA rings were to get cut. The insulin gene and the plasmid were then “spliced” together. We then tested to see if the plasmid was taken into the host bacteria cells. An example would be ampicillin, as it is an antibiotic that is usually able to kills bacterial cells.


  1. Tetracycline, kanamycin, and ampicillin. I would use these three because these usually kill the host bacteria.
  2. The restriction enzyme cuts open the plasmid at one site. This is because the DNA will be inserted there. We used Eco R1 and Hin dIII because they were on both of the human genes twice.
  3. The DNA won’t be able to be inserted into the plasmid.
  4. This process is important in everyday life because this prevents us from contracting illnesses and diseases that would harm us.
  5. This process could be used to increase the amount of eggs a chicken produces. This way, we can breed less chickens, kill less chickens, and still get the same amount of eggs. Either that, or we could keep less chickens penned up and only have a few produce a large amount of eggs.

Monday, January 4, 2016

New Years Goals

New Years Goals
My new years goal for biology is concise. Continue doing what I have been doing in class, except be more in the class. What I mean by “in the class” is to pay attention, not only to what Mr. Orre is saying, but also pay attention to my surroundings. Are my classmates paying attention? What part of the book is he talking about right now? Does this part deserve attention or do I already know this thoroughly? If I can improve on these few things, then I will be able to achieve better understanding and a higher grade in this class.


My new years goal for golf is much different than biology. Paying attention to surroundings can matter, but most of the time I will try to zone out, paying attention to mostly my game and my game only. Other’s game I will give no attention towards, and if I stray from my goal I will try to redirect my course to where I want to be. Getting to the top 8, or being a traveler should be a stepping stone. My goal is to place in the top 4, where I feel I can be if i devote enough time and work to short game and my irons. Accuracy is key on Saratoga Country Club, and if I continue to scramble successfully and hit the greens, I can secure a spot in the top 4 for myself.

Sunday, December 27, 2015

Unit 5 Reflection/ Notes
Unit 5 was the unit based off of… mutations. Problems with DNA, sometimes good, sometimes harmless, and sometimes detrimental. This unit went over point mutations, frameshift mutations, inversions, and translocations.
Point mutations are changes in one or two base pairs of DNA, and are small but common. These can be harmful if the protein changes a lot, but if the protein doesn’t really change they’re harmless. There are two main types of point mutations; substitution and frameshift mutations. In substitution, a nucleotide is substituted for another(ex. T is replaced by A by mistake). Frameshift mutations occur when a, insertion, where an extra base pair is added into the code, or b, deletion, where a base pair is left out of the code.

Some other mutations include inversion, where a piece of DNA breaks off and bonds in reverse order. Translation occurs when part of a chromosome breaks off and bonds with another chromosome.

Why do mutations matter to us though? First off, changes in DNA means changes in proteins, and some diseases are caused by the smallest of things, even a change of a single base. Mutations can be detrimental like said earlier, resulting in cancers or life threatening problems. Mutations can be very useful too. They could accidentally create new genetic variation for natural selection, causing resistances to certain things in nature.
Gene regulation is the reason why we don’t sprout hair on our eyes and ears on our chest. Cells don’t want to perform more than the necessary actions for the cell to function, so there is something called an operator that prevents the RNA polymerase from reading the gene. This is necessary, because without the operator the RNA polymerase would read the gene and keep making excess amounts of whatever results from the initiated process.

Unit 4 Notes
Unit 4 consisted of many steps and processes, all related to cells and development.  
  • DNA fingerprinting- analyzes sections of DNA that have little to no known function but vary widely from one individual to another.     
  • Gene theory- whenever there is an absent or faulty gene, it is replaced by a normal and working gene.
  • The Human Genome Project- ongoing effort to analyze human DNA sequence.
Punnett squares are extremely useful  charts, made to draw possible phenotypes of an offspring based upon its parents’ genes.
Mendel’s work showed people the reason why we all look different, and why we all have different traits. His work taught us about dominance, recessiveness, and variation.
Two types of Inheritance: Autosomal and x-linked.
The Law of Segregation- Gene pairs for a trait separate when gametes are formed

Unit 3 Notes
Cells are called cells because a scientist, Robert Hooke, used an early compound microscope to look at a thin slice of cork. This cork showed many little chambers, almost as if they were “cells”. Hooke called these little chambers cells and they are known as cells to the present day. He used this microscope to look at other things, and found out that everything he looked at had “cells” as well.
The cell supports itself using different processes. Some of these processes create new organelles, some exterminate old, unproductive organelles, and some let things in and out of the cell.
Prokaryotes are cells with no nucleus, and lack many organelles compared to a eukaryote, which has all of those.
Unit 2 Notes
Photosynthesis is a process a plant cell undergoes to create ATP for the cell to work and for the plant to be supplied with energy. It converts energy from the sun and water from the roots in the soil to ATP using photosynthesis, which occurs in the chloroplast unique to plant cells.

Nucleus- nucleolus
Ribosome- small subunit(bottom), large subunite(top), builds proteins
Vesicle- membrane bound container
Rough ER- ribosome son the outside, “factory”
Golgi body- add stuff to proteins then “ships” the proteins
Cytoskeleton- structure
smooth ER- produces lipids, cholesterol, etc, detoxification
Mitochondria- produce atp, have own DNA
Vacuole- in plants, stores stuff
Cytosol- fluid that contains solutes, concentration gradient
Lysosome- Digestive enzymes, “suicide”
Centriole- important in positioning, initiate formation of spindle

Unit 1 Notes
Scientific method
  1. Question/ problem
  2. Collect information
  3. Hypothesis
  4. Experiment
  5. Results/ data
  6. Analysis/ conclusion(support/reject)
Qualitative- Descriptions
Quantitative- Numbers
Positive control- a group of data that will give predictable positive results
Negative control- A group of data lacking what is being tested so as to give expected negative results
Control- Something you use to compare your results to
Independent Variable- something that will change
Dependent Variable- something that will be measured
Constant- remains the same throughout experiment



Vocabulary
  • Mutations- a change in the DNA code
  • Mutagen- anything that causes a mutation
  • Gene expression- process of a gene being used to produce a gene product or phenotype
  • Gene regulation- mechanism used by cells to increase or decrease the expression of a gene
  • Exons- sequences that are expressed
  • Introns- sequences that are cut out
  • Codominance- both traits are visible
  • Incomplete dominance- Both partially show


Monday, December 14, 2015

Unit 5 Reflection

Unit 5 Reflection/ Notes
Unit 5 was the unit based off of… mutations. Problems with DNA, sometimes good, sometimes harmless, and sometimes detrimental. This unit went over point mutations, frameshift mutations, inversions, and translocations.
Point mutations are changes in one or two base pairs of DNA, and are small but common. These can be harmful if the protein changes a lot, but if the protein doesn’t really change they’re harmless. There are two main types of point mutations; substitution and frameshift mutations. In substitution, a nucleotide is substituted for another(ex. T is replaced by A by mistake). Frameshift mutations occur when a, insertion, where an extra base pair is added into the code, or b, deletion, where a base pair is left out of the code.

Some other mutations include inversion, where a piece of DNA breaks off and bonds in reverse order. Translation occurs when part of a chromosome breaks off and bonds with another chromosome.

Why do mutations matter to us though? First off, changes in DNA means changes in proteins, and some diseases are caused by the smallest of things, even a change of a single base. Mutations can be detrimental like said earlier, resulting in cancers or life threatening problems. Mutations can be very useful too. They could accidentally create new genetic variation for natural selection, causing resistances to certain things in nature.
Gene regulation is the reason why we don’t sprout hair on our eyes and ears on our chest. Cells don’t want to perform more than the necessary actions for the cell to function, so there is something called an operator that prevents the RNA polymerase from reading the gene. This is necessary, because without the operator the RNA polymerase would read the gene and keep making excess amounts of whatever results from the initiated process.

Friday, December 4, 2015

Human DNA Extraction Lab

Human DNA Extraction Lab

“How can DNA be separated from cheek cells to study it?” That was the question we stated our claim off of, which was homogenization, lysis, and precipitation. We swished gatorade around in our mouths to fill it with cheek cells, and after that we put salt, the enzyme for this, and dish soap. After this, we slowly poured alcohol into the test tube, and the DNA slowly diffused into the alcohol. This data supports our claim because it follows homogenization, lysis, precipitation and it allows us to study the DNA.
Our data agrees with the expected results because our steps, if followed correctly, were supposed to yield some DNA in the alcohol, which is what happened. There could have been errors with exact drop measurements, which could allow for too little DNA or the alcohol might not have separated from the soapy gatorade. There also could have been mistiming on how long we let the gatorade sit for, which might have limited the amount of DNA that came out. We could have solved these problems by using more precise measurements with drops, and by starting the timer exactly when we spit it out.

This lab was done to demonstrate the process used to collect DNA from a sample. From this lab I learned how to collect DNA from a sample, and this can help people in real life because of investigations. Based on my experience from this lab, I will be able to produce real DNA samples in the future, and DNA is so much more interesting to me now.


Wednesday, November 18, 2015

Coin Sex Conclusion

In the coin sex lab, we flipped coins to determine alleles for a sample set, then analyzed the data and answered some questions. We were expecting to receive 5 heterozygous and 5 homozygous for every 10 coins flipped, because there was a 50% chance of receiving either one. As it turns out, we didn’t get 50% of both because on our first set of flips, we got 6 females and 4 males rather than 5 females and 5 males.
The limit of using probability to predict the offspring’s trait is the amount of tests that we conduct. If we do 10 flips, but we get 9 females and 1 male, that’s not very accurate. If we do 40 more, maybe we will get around 25 males and 15 females, making the scales a bit more balanced. Some gene interactions are x-linked, so even if we get more females sometimes, the males are the only ones that can receive the traits.

This lab relates to my life because knowing my mom and dad’s traits could help me determine what mine could be, or maybe a future sibling(probably not).

Genetics Infographic

Published version of my infographic:
https://magic.piktochart.com/output/9065894-bachelors-of-science-in-science-history