pGLO Observations

1.

Plate	Number of Colonies	Color of Colonies under room light	Color of colonies under UV light
-pGLO LB	carpet	dark yellow	slightly purple/ dark yellow
-pGLO LB/amp	none	-	-
+pGLO LB/amp	150-200	dark yellow	slightly purple/ dark yellow
+pGLo LB/amp/ara	47	dark yellow	glowing green

2. One glows under the presence of UV light. One multiplied in colony size greatly, and one didn’t have any colonies at all.

3. There were 47 colonies for the plate with arabinose(counted), and the one with no plasmid but LB had an uncountable amount of colonies, resulting in “carpet”. The plate with no plasmid but had LB and ampicillin had no colonies at all, while the plate with the plasmid, LB, and ampicillin had 150-200 colonies of bacteria.

4. Arabinose is the sugar that essentially turns on and makes GFP glow green and is also an alternative carbon source for E.coli.

5. To test and see if inserting genes into organisms will work.

GFP shows us when proteins are made.

Researching why GFP developed in the Aequorea victoria jellyfish.

6. A) Engineering chickens to be fatter and bigger in order to sell more chicken.
B) Genetically altered grapes that are larger.